How to Prepare Alkaline Lysis Solution II (P2)
Alkaline lysis buffer for plasmid miniprep. Prepare fresh
Components
| Chemical | Concentration |
|---|---|
| NaOH | 200 mM |
| SDS | 1% w/v |
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TE Buffer
Standard DNA/RNA storage buffer
6X DNA Loading Dye
6X concentrated gel loading dye for agarose gel electrophoresis
20X SSC Buffer
Standard hybridization and wash buffer for Southern/Northern blots
Church Hybridization Buffer
Rapid hybridization buffer for Southern/Northern blots
DEPC-Treated Water
RNase-free water for RNA work, autoclave after DEPC treatment to decompose residual DEPC
10X MOPS Running Buffer
10X stock for denaturing RNA agarose gel electrophoresis