How to Prepare TBST Wash Buffer
TBS with 0.1% Tween 20, standard Western blot wash buffer
Components
| Chemical | Concentration |
|---|---|
| Tris base | 20 mM (pH 7.6) |
| NaCl | 150 mM |
| Tween 20 | 0.1% v/v |
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PBST (PBS-Tween 20)
PBS with 0.1% Tween 20, standard wash buffer for ELISA and Western blot
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Tris-HCl Buffer pH 8.0
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