How to Prepare Western Blot Stripping Buffer (Harsh)
Harsh stripping at 50-60C for difficult-to-remove antibodies, may damage membrane
Components
| Chemical | Concentration |
|---|---|
| Tris-HCl | 62.5 mM (pH 6.8) |
| SDS | 2% w/v |
| 2-Mercaptoethanol | 100 mM |
Need a different volume or concentration?
Customize this solutionRelated Solutions
Western Blot Blocking Buffer (BSA)
5% BSA in TBST for Western blot membrane blocking, recommended for phospho-protein detection
Western Blot Blocking Buffer (Milk)
5% non-fat milk in TBST for Western blot blocking, do NOT use for phospho-protein detection
Western Blot Stripping Buffer (Mild)
Mild stripping buffer to remove primary/secondary antibodies from Western blot membranes
Ponceau S Staining Solution
Reversible protein stain for PVDF/nitrocellulose membranes, used to verify transfer efficiency