How to Prepare Western Blot Stripping Buffer (Mild)
Mild stripping buffer to remove primary/secondary antibodies from Western blot membranes
Components
| Chemical | Concentration |
|---|---|
| Glycine | 200 mM (pH 2.2) |
| SDS | 0.1% w/v |
| Tween 20 | 1% v/v |
Need a different volume or concentration?
Customize this solutionRelated Solutions
Western Blot Blocking Buffer (BSA)
5% BSA in TBST for Western blot membrane blocking, recommended for phospho-protein detection
Western Blot Blocking Buffer (Milk)
5% non-fat milk in TBST for Western blot blocking, do NOT use for phospho-protein detection
Western Blot Stripping Buffer (Harsh)
Harsh stripping at 50-60C for difficult-to-remove antibodies, may damage membrane
Ponceau S Staining Solution
Reversible protein stain for PVDF/nitrocellulose membranes, used to verify transfer efficiency